Gram staining is a common method used to differentiate between two groups of bacteria depending on their different cell wall structure. To remember it .. mnemonics
LONG is related to gm -ve and PPT related to gm +ve
What is the difference then in the cell wall structure ?
Gram +ve VS gram -ve
Stain blue or purple
Stain red or rose pink
Lipopolysaccharide (LPS) in outer membrane
Peptidoglycan layer is thick
Peptidoglycan layer is thin
Exotoxin is a metabolic product
Endotoxin is a metabolic product
Exotoxin is thermolabile (heat sensitive) destroyed at a temperature over 60 oC
Endotoxin is more toxic than exotoxin, destroyed at higher temperatures.
Exotoxin is a high molecular weight protein.
Endotoxin a complex structure made up of phospholipid, polysaccharides and protein.
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